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crystal violet solution  (Beyotime)


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    Structured Review

    Beyotime crystal violet solution
    Crystal Violet Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 6635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/Crystal+Violet+Staining+Solution/pmc13020017-138-1-4
    Average 99 stars, based on 6635 article reviews
    crystal violet solution - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Liquiritin Enhances Chemosensitivity to Doxorubicin in Breast Cancer by Promoting Ubiquitination-Mediated Degradation of MCL1.
    Article Snippet: Breast cancer (BC) represents a life‐threatening malignant disease that profoundly endangers women's health, with chemoresistance restricting treatment efficacy.. Liquiritin (LIQ) has shown great potential in cancer therapy, yet its effect on the chemosensitivity of BC cells remains unclear.. BC cells were treated with different concentration gradients of Doxorubicin (DOX) and LIQ.

    Article Title: Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma
    Article Snippet: .. Dead or apoptotic cells in the organoids were stained using Beyo3DTM YO-PRO-1 (C1356S, Beyotime, China) staining solution, following the manufacturer's instructions. .. Six organoids were randomly selected from each group to analyze the ratio of dead cell area to the total organoid area using ImageJ software.

    Article Title: Discovery of the late autophagy inhibitor FZU-0045-053 and its anti-breast cancer and immunomodulatory effects
    Article Snippet: .. In the present study, the cells were treated with 053 (0, 5 or 10 μ M), DOC (10 μ M), DDP (50 μ M), GEM (80 μ M), PTX (0.1 μ M) and 5-FU (50 μ M) alone or combined with 053 (5 μ M) for 24 h. Subsequently, the cells were collected and washed with Annexin V-FITC binding buffer (cat. no. C1062S; Beyotime Biotechnology) twice, resuspended with staining solution containing 5 μ l Annexin V-FITC and 5 μ l PI (cat. no. C1062S; Beyotime Biotechnology) and incubated for 30 min in the dark. ..

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Article Title: Przewaquinone A regulates cell cycle and autophagy through the SrC/STAT3 signaling pathway to inhibit colorectal cancer progression.
    Article Snippet: Przewaquinone A (PrA), a natural active substance extracted from Salvia przewalskii Maxim, has been shown to have antitumor activity and can act as a STAT3 inhibitor to modulate the Src/STAT3 pathway.. However, its role in colorectal cancer (CRC), along with its related mechanisms, has not yet been clarified.. This study aims to explore whether PrA inhibits the malignant advancement of CRC via the Src/STAT3 pathway, and to provide new drug candidates and molecular targets for CRC clinical treatment.

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)

    Article Title: Inhibiting Arachidonate-5-lipoxygenase expression ameliorates osteoarthritis progression by suppressing ferroptosis via the JAK2/STAT3 signaling pathway.
    Article Snippet: Background: Osteoarthritis (OA) is a degenerative joint disease.. Recent studies have shown that ferroptosis plays a critical role in OA.. Arachidonate-5-lipoxygenase (ALOX5), a pivotal enzyme regulating arachidonic acid metabolism, is involved in the synthesis of the pro-inflammatory leukotrienes.

    Binding Assay:

    Article Title: Discovery of the late autophagy inhibitor FZU-0045-053 and its anti-breast cancer and immunomodulatory effects
    Article Snippet: .. In the present study, the cells were treated with 053 (0, 5 or 10 μ M), DOC (10 μ M), DDP (50 μ M), GEM (80 μ M), PTX (0.1 μ M) and 5-FU (50 μ M) alone or combined with 053 (5 μ M) for 24 h. Subsequently, the cells were collected and washed with Annexin V-FITC binding buffer (cat. no. C1062S; Beyotime Biotechnology) twice, resuspended with staining solution containing 5 μ l Annexin V-FITC and 5 μ l PI (cat. no. C1062S; Beyotime Biotechnology) and incubated for 30 min in the dark. ..

    Incubation:

    Article Title: Discovery of the late autophagy inhibitor FZU-0045-053 and its anti-breast cancer and immunomodulatory effects
    Article Snippet: .. In the present study, the cells were treated with 053 (0, 5 or 10 μ M), DOC (10 μ M), DDP (50 μ M), GEM (80 μ M), PTX (0.1 μ M) and 5-FU (50 μ M) alone or combined with 053 (5 μ M) for 24 h. Subsequently, the cells were collected and washed with Annexin V-FITC binding buffer (cat. no. C1062S; Beyotime Biotechnology) twice, resuspended with staining solution containing 5 μ l Annexin V-FITC and 5 μ l PI (cat. no. C1062S; Beyotime Biotechnology) and incubated for 30 min in the dark. ..

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Article Title: Inhibiting Arachidonate-5-lipoxygenase expression ameliorates osteoarthritis progression by suppressing ferroptosis via the JAK2/STAT3 signaling pathway.
    Article Snippet: Background: Osteoarthritis (OA) is a degenerative joint disease.. Recent studies have shown that ferroptosis plays a critical role in OA.. Arachidonate-5-lipoxygenase (ALOX5), a pivotal enzyme regulating arachidonic acid metabolism, is involved in the synthesis of the pro-inflammatory leukotrienes.

    CCK-8 Assay:

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Infection:

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Microscopy:

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)



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    Servicebio Inc ros staining solution
    Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans <t>blue</t> <t>staining</t> to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of <t>ROS</t> and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.
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    Image Search Results


    Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

    Journal: Redox Biology

    Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

    doi: 10.1016/j.redox.2026.104198

    Figure Lengend Snippet: Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

    Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

    Techniques: Staining, Expressing, Transmission Assay, Electron Microscopy, Microscopy, Control

    Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

    Journal: Redox Biology

    Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

    doi: 10.1016/j.redox.2026.104198

    Figure Lengend Snippet: Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

    Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

    Techniques: Expressing, Staining, Transmission Assay, Electron Microscopy, Microscopy, Control